RNDr. Radek Šíma, Ph.D.

Job Position: Research Scientist - Laboratory of Molecular Biology of Ticks

Contact details

Phone: +420 387775496
E-mail: sima@paru.cas.cz
Room: 308

Description

Research interests

Molecular mechanisms of the vector parasite interactions. Laboratory models of the most important tick borne diseases as an essential tools to study vaccine candidates.

Curriculum vitae

Date of birth: 20.6. 1978

Academic history

• 1996-2002 Faculty of Biology (Faculty of Science), University of South Bohemia, Ceske Budejovice
  • 1999: BSc. graduated. Specialization: Biochemistry of proteins, microbiology.
    Diploma work: Lecitin of spirochaeta Borrelia burgdorferi – Isolation and partial characterisation.
  • 2002: MSc. graduated. Specialization: Biochemistry of proteins, molecular biology, immunology.
    Diploma work: Structural and functional characterisation of a lectin of spirochaeta Borrelia burgdorferi.
  • 2007: RNDr. graduated. Specialization: Molecular biology and genetics of tumors.
    Diploma work: Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple.
• 2006-2010: Charles University, Medical faculty in Pilsen
  • 2010: PhD. graduated. Specialization: Molecular and genetic characterisation of tumors.
    Doctoral thesis: Correlation of morphologic and molecular-genetic features of selected tumors.

Employment

• 1999-2002: Institute of Parasitology, Laboratory of Molecular Biology of Vector and Pathogens, Czech Academy of Sciences, Ceske Budejovice. Field of study: Molecular mechanisms of the vector parasite interactions.
• 2002-2011: Sikl’s Department of Pathology, Charles University, Plzen
  Field of study: Molecular mechanisms of the tumorigenesis, molecular pathogenesis of the infectious and genetic diseases.
• 2011 till present: Institute of Parasitology - Laboratory of Vector Immunology, Czech Academy of Sciences, Ceske Budejovice
  Field of study: Molecular mechanisms of the vector parasite interactions. Laboratory models of the most important tick borne diseases as an essential tools to study vaccine candidates.

Publications

2003-2011 45 publications in journals with impact factor.

• 1. Hes O, Hora M, Vanecek T, Sima R, Sulc M, Havlicek F, Beranova M, Michal M. Conventional renal cell carcinoma with granulomatous reaction: a report of three cases. Virchows Arch. 2003 Aug;443(2):220-1.
• 2. Boudova L, Vanecek T, Sima R, Bouda J, Hes O, Fakan F. Role of p27Kip1 protein in the cell cycle and its appearance in lymphoid tissues, particularly non-Hodgkin's B-cell lymphomas. (Review). Cesk Patol. 2003 Jul;39(3):126-9.
• 3. Daum O, Hes O, Vanecek T, Benes Z, Sima R, Zamecnik M, Mukensnabl P, Hadravska S, Curik R, Michal M. Vanek's tumor (inflammatory fibroid polyp). Report of 18 cases and comparison with three cases of original Vanek's series. Ann Diagn Pathol. 2003 Dec;7(6):337-47.
• 4. Michal M, Vanecek T, Sima R, Mukensnabl P, Boudova L, Brouckova M. Primary capillary hemangioblastoma of peripheral soft tissues. Am J Surg Pathol. 2004 Jul;28(7):962-6.
• 5. Daum O, Vanecek T, Sima R, Curik R, Zamecnik M, Yamanaka S, Mukensnabl P, Benes Z, Michal M. Reactive nodular fibrous pseudotumors of the gastrointestinal tract: report of 8 cases. Int J Surg Pathol. 2004 Oct;12(4):365-74.
• 6. Kazakov DV, Pitha J, Sima R, Vanecek T, Shelekhova K, Mukensnabl P, Michal M. Hybrid peripheral nerve sheath tumors: Schwannoma-perineurioma and neurofibroma-perineurioma. A report of three cases in extradigital locations. Ann Diagn Pathol. 2005 Feb;9(1):16-23.
• 7. Kazakov DV, Hes O, Hora M, Sima R, Michal M. Primary intranodal cellular angiolipoma. Int J Surg Pathol. 2005 Jan;13(1):99-101.
• 8. Hes O, Benakova K, Vanecek T, Sima R, Michal M. Clear cell type of renal cell carcinoma with numerous hyaline globules: a diagnostic pitfall. Pathol Int. 2005 Mar;55(3):150-4.
• 9. Daum O, Klecka J, Ferda J, Treska V, Vanecek T, Sima R, Mukensnabl P, Michal M. Gastrointestinal stromal tumor of the pancreas: case report with documentation of KIT gene mutation. Virchows Arch. 2005 Apr;446(4):470-2.
• 10. Daum O, Sima R, Mukensnabl P, Vanecek T, Brouckova M, Michal M. Pigmented Solid-Pseudopapillary Neoplasm of the Pancreas. Pathol Int. 2005 May;55(5):280-4.
• 11. Hes O, Vanecek T, Sima R, Hora M, Velickinova H, Grossmann P, Kovar J, Michal M. Tumorous diseases in patients with the testicular feminization syndrome ("androgen insensitivity" syndrome) - description of two cases. Ceska Gynekol. 2005 Mar;70(2):113-7.
• 12. Kazakov DV, Sima R, Michal M. Hemosiderotic fibrohistiocytic lipomatous lesion: clinical correlation with venous stasis. Virchows Arch. 2005 Jul;447(1):103-6.
• 13. Kazakov DV, Vanecek T, Sima R, Kutzner H, Michal M. Dendritic cell neurofibroma with pseudorosettes lacks mutations in exons 1-15 of the neurofibromatosis type 2 gene. Am J Dermatopathol. 2005 Aug;27(4):286-9.
• 14. Boudova L, Kazakov DV, Sima R, Vanecek T, Torlakovic E, Lamovec J, Kutzner H, Szepe P, Plank L, Bouda J, Hes O, Mukensnabl P, Michal M. Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of 56 patients. Am J Dermatopathol. 2005 Oct;27(5):375-86.
• 15. Kazakov DV, Bisceglia M, Sima R, Michal M. Adenosis tumor of anogenital mammary-like glands: a case report and demonstration of clonality by HUMARA assay. J Cutan Pathol. 2006 Jan;33(1):43-6.
• 16. Kinkor Z, Sima R, Skalova A, Boudova L, Pekova S, Dvorakova D, Dedic K, Kracik M, Janousek M, Michal M. Isolated lymphadenopathy as the first presentation of systemic mastocytosis--description of two cases Cesk Patol. 2006 Jan;42(1):34-8.
• 17. Michal M, Vanecek T, Sima R, Mukensnabl P, Hes O, Kazakov DV, Matoska J, Zuntova A, Dvorak V, Talerman A. Mixed germ cell sex cord-stromal tumors of the testis and ovary. Morphological, immunohistochemical, and molecular genetic study of seven cases. Virchows Arch. 2006 May;448(5):612-22.
• 18. Kralickova M, Sima R, Vanecek T, Sima P, Rokyta Z, Ulcova-Gallova Z, Sucha R, Uher P, Hes O. Leukemia inhibitory factor gene mutations in the population of infertile women are not restricted to nulligravid patients. Eur J Obstet Gynecol Reprod Biol. 2006 2006 Aug;127(2):231-5
• 19. Skalova A, Gnepp DR, Simpson RH, Lewis JE, Janssen D, Sima R, Vanecek T, Di Palma S, Michal M. Clonal nature of sclerosing polycystic adenosis of salivary glands demonstrated by using the polymorphism of the human androgen receptor (HUMARA) locus as a marker. Am J Surg Pathol. 2006 Aug;30(8):939-44.
• 20. Boudova L, Kazakov DV, Jindra P, Sima R, Vanecek T, Kuntscher V, Vera V, Bouda J, Michal M. Primary cutaneous histiocyte and neutrophil-rich CD30+ and CD56+ anaplastic large-cell lymphoma with prominent angioinvasion and nerve involvement in the forehead and scalp of an immunocompetent woman. J Cutan Pathol. 2006 Aug;33(8):584-9.
• 21. Kazakov DV, Belousova IE, Sima R, Michal M. Mammary type tubulolobular carcinoma of the anogenital area: report of a case of a unique tumor presumably originating in anogenital mammarylike glands. Am J Surg Pathol. 2006 Sep;30(9):1193-6.
• 22. Daum O, Grossmann P, Vanecek T, Sima R, Mukensnabl P, Michal M. Diagnostic morphological features of PDGFRA-mutated gastrointestinal stromal tumors: molecular genetic and histologic analysis of 60 cases of gastric gastrointestinal stromal tumors. Ann Diagn Pathol. 2007 Feb;11(1):27-33.
• 23. Belousova IE, Nikonova SM, Sima R, Kazakov DV. Granulomatous slack skin with clonal T-cell receptor-gamma gene rearrangement in skin and lymph node. Br J Dermatol. 2007 Aug;157(2):405-7.
• 24. Kazakov DV, Bisceglia M, Spagnolo DV, Kutzner H, Belousova IE, Hes O, Sima R, Vanecek T, Yang Y, Michal M. Apocrine Mixed Tumors of the Skin With Architectural and/or Cytologic Atypia: A Retrospective Clinicopathologic Study of 18 Cases. Am J Surg Pathol. 2007 Jul;31(7):1094-1102.
• 25. Michal M, Kazakov DV, Síma R, Vanecek T. Primitive small cell tumor with epithelial, gangliocytic, neuroendocrine, and mesenchymal differentiation: report of 2 cases. Int J Surg Pathol. 2007 Oct;15(4):429-36.
• 26. Králíčková M, Síma R, Martínek P, Vanecek T, Ulcová-Gallová Z, Síma P, Krizan J, Kalis V, Stepán Jr, Stepán J, Rokyta Z, Uher P, Hes O. The leukemia inhibitory factor gene mutations in the population of infertile women: the heterozygote transition G to A on the position 3400 does not affect the outcome of the infertility treatment. Ceska Gynekol. 2007 Aug;72(4):293-8.
• 27. Hes O, Michal M, Síma R, Vaněček T, Brunelli M, Martignoni G, Kuroda N, Alvarado Cabrero I, Perez-Montiel D, Hora M, Urge T, Dvořák M, Jarošová M, Yang X. Renal oncocytoma with and without intravascular extension into the branches of renal vein have the same morphological, immunohistochemical, and genetic features. Virchows Arch. 2008 Feb;452(2):193-200.
• 28. Králícková M, Ulcová-Gallová Z, Síma R, Vanecek T, Síma P, Krizan J, Suchá R, Uher P, Hes O, Novotný Z, Rokyta Z, Vetvicka V. Association of the leukemia inhibitory factor gene mutation and the antiphospholipid antibodies in the peripheral blood of infertile women. Folia Microbiol (Praha). 2007;52(5):543-8.
• 29. Vazmitel M, Michal M, Shelekhova KV, Sima R, Mukensnabl P, Kazakov DV. Vascular changes in merkel cell carcinoma based on a histopathological study of 92 cases. Am J Dermatopathol. 2008 Apr;30(2):106-11.
• 30. Skalova A, Síma R, Bohuš P, Cuřík R, Lukáš J, Michal M. Endolymphatic sac tumor (aggressive papillary tumor of middle ear and temporal bone) Report of two cases with analysis of the VHL gene. Pathol Res Pract. 2008;204(8):599-606.
• 31. Kazakov DV, Belousova IE, Kacerovska D, Sima R, Vanecek T, Vazmitel M, Pizinger K, Michal M. Hyperplasia of hair follicles and other adnexal structures in cutaneous lymphoproliferative disorders: a study of 53 cases, including so-called pseudolymphomatous folliculitis and overt lymphomas. Am J Surg Pathol. 2008 Oct;32(10):1468-78.
• 32. Hes O, Síma R, Nemcová J, Hora M, Bulimbasic S, Kazakov DV, Urge T, Reischig T, Dvorák M, Michal M. End-stage kidney disease: gains of chromosomes 7 and 17 and loss of Y chromosome in non-neoplastic tissue. Virchows Arch. 2008 Oct;453(4):313-9.
• 33. Shelekhova KV, Sima R, Orlov AIu, Kazakov DV, Michal M. Intraneural perineurioma: clinical and morphological characteristics and a molecular genetic study of the neurofibromatosis type 2 gene. Arkh Patol. 2008 Jul-Aug;70(4):20-2.
• 34. Michal M, Hes O, Nemcova J, Sima R, Kuroda N, Bulimbasic S, Franco M, Sakaida N, Danis D, Kazakov DV, Ohe C, Hora M. Renal angiomyoadenomatous tumor: morphologic, immunohistochemical, and molecular genetic study of a distinct entity. Virchows Arch. 2009 Jan;454(1):89-99.
• 35. Kazakov DV, Zelger B, Rütten A, Vazmitel M, Spagnolo DV, Kacerovska D, Vanecek T, Grossmann P, Sima R, Grayson W, Calonje E, Koren J, Mukensnabl P, Danis D, Michal M. Morphologic Diversity of Malignant Neoplasms Arising in Preexisting Spiradenoma, Cylindroma, and Spiradenocylindroma Based on the Study of 24 Cases, Sporadic or Occurring in the Setting of Brooke-Spiegler Syndrome. Am J Surg Pathol. 2009 May;33(5):705-19.
• 36. Kazakov DV, Ivan D, Kutzner H, Spagnolo DV, Grossmann P, Vanecek T, Sima R, Kacerovska D, Shelekhova KV, Denisjuk N, Hillen U, Kuroda N, Mukensnabl P, Danis D, Michal M. Cutaneous hidradenocarcinoma: a clinicopathological, immunohistochemical, and molecular biologic study of 14 cases, including Her2/neu gene expression/amplification, TP53 gene mutation analysis, and t(11;19) translocation. Am J Dermatopathol. 2009 May;31(3):236-47.
• 37. Kazakov DV, Sima R, Vanecek T, Kutzner H, Palmedo G, Kacerovska D, Grossmann P, Michal M. Mutations in exon 3 of the CTNNB1 gene (beta-catenin gene) in cutaneous adnexal tumors. Am J Dermatopathol. 2009 May;31(3):248-55.
• 38. Bulimbasic S, Ljubanovic D, Sima R, Michal M, Hes O, Kuroda N, Persec Z. Aggressive high-grade mucinous tubular and spindle cell carcinoma. Hum Pathol. 2009 Jun;40(6):906-7.
• 39. Skálová A, Sima R, Vanecek T, Muller S, Korabecna M, Nemcova J, Elmberger G, Leivo I, Passador-Santos F, Walter J, Rousarova M, Jedlickova K, Curik R, Geierova M, Michal M. Acinic Cell Carcinoma With High-grade Transformation: A Report of 9 Cases With Immunohistochemical Study and Analysis of TP53 and HER-2/neu Genes. Am J Surg Pathol. 2009 May 20.
• 40. Kacerovska D, Sima R, Michal M, Hes O, Roucka P, Zarybnicka M, Hora M, Chudacek Z, Kazakov DV. Carney complex: a clinicopathologic and molecular biological study of a sporadic case, including extracutaneous and cutaneous lesions and a novel mutation of the PRKAR1A gene. J Am Acad Dermatol. 2009 Jul;61(1):80-7.
• 41. Novotný Z, Krízan J, Síma R, Síma P, Uher P, Zech N, Hütelová R, Baborová P, Ulcová-Gallová Z, Subrt I, Ulmanová E, Houdek Z, Rokyta Z, Babuska V, Králícková M. Leukaemia inhibitory factor (LIF) gene mutations in women diagnosed with unexplained infertility and endometriosis have a negative impact on the IVF outcome. A pilot study. Folia Biol (Praha). 2009;55(3):92-7.
• 42. Yamaguchi T, Kuroda N, Imamura Y, Hes O, Michal M, Sima R, Nakayama K, Sato N. Imprint cytologic features of chromophobe renal cell carcinoma morphologically resembling renal oncocytoma: Is this an oncocytic variant of chromophobe renal cell carcinoma? Diagn Cytopathol. 2010 Jul;38(7):509-13.
• 43. Petersson F, Bulimbasic S, Sima R, Michal M, Hora M, Malagon HD, Matoska J, Hes O. Large cell calcifying Sertoli cell tumor: a clinicopathologic study of 1 malignant and 3 benign tumors using histomorphology, immunohistochemistry, ultrastructure, comparative genomic hybridization, and polymerase chain reaction analysis of the PRKAR1A gene. Hum Pathol. 2010 Apr;41(4):552-9. Epub 2009 Dec 11.
• 44. Sima R, Vanecek T, Kacerovska D, Trubac P, Cribier B, Rutten A, Vazmitel M, Spagnolo DV, Litvik R, Vantuchova Y, Wyers W, Pearce RL, Pearn J, Michal M, Kazakov DV. Brooke-Spiegler syndrome: report of 10 patients from 8 families with novel germline mutations. Evidence of diverse somatic mutations in the same patient regardless of tumor type. Diagn Mol Pathol. 2010 Jun;19(2):83-91.
• 45. Skalova A, Vanecek T, Sima R, Laco J, Weinreb I, Perez-Ordonez B, Starek I, Geierova M, Simpson RHW, Passador-Santos F, Ryska A, Leivo I, Kinkor Z, Michal M. Mammary analogue secretory carcinoma of salivary glands, containing the ETV6-NTRK3 fusion gene: a hitherto undescribed salivary gland tumor entity. Am J Surg Pathol. 2010 May;34(5):599-608.
• 46. Kuroda N, Hosokawa T, Michal M, Hes O, Sima R, Ohe C, Lee GH. Clear cell renal cell carcinoma with focal renal angiomyoadenomatous tumor-like area. Ann Diagn Pathol. 2010 Jun 16.
• 47. Petersson F, Síma R, Sperga M, Kazakov DV, Michal M, Hora M, Ferda J, Sulc M, Mičulka P, Haferník J, Rychnovský J, Hes O. Lymphocyte-rich renal cell carcinoma: an unusual histomorphologic manifestation of a tumor that is not part of lynch syndrome. Appl Immunohistochem Mol Morphol. 2011 Dec;19(6):519-27.
• 48. Ondic O, Kalis V, Sima R. Borderline papillary serous tumor of the fimbriated end of the fallopian tube with peritoneal implants. J Obstet Gynaecol Res. 2011 Nov;37(11):1702-5.
• 49. Petersson F, Síma R, Grossmann P, Michal M, Kuroda N, Hora M, Yang X, Kinkor Z, Trivunic S, Zalud R, Sperga M, Jaunmuktane Z, Branžovský J, Ferda J, Hes O. Renal small cell oncocytoma with pseudorosettes A histomorphologic, immunohistochemical, and molecular genetic study of 10 cases. Hum Pathol. 2011 Nov;42(11):1751-60.
• 50. Skalova A, Sima R, Kaspirkova-Nemcova J, Simpson RH, Elmberger G, Leivo I, Di Palma S, Jirasek T, Gnepp DR, Weinreb I, Perez-Ordoñez B, Mukensnabl P, Rychly B, Hrabal P, Michal M. Cribriform adenocarcinoma of minor salivary gland origin principally affecting the tongue: characterization of new entity. Am J Surg Pathol. 2011 Aug;35(8):1168-76.
• 51. Dubská L, Vyskocilová M, Nenutil R, Valík D, Knoflícková D, Fabian P, Kocáková I, Demlová R, Beránek M, Drastíková M, Vosmiková H, Bóday A, Horká K, Símová J, Drábek J, Ehrmann J, Hajdúch M, Matejcková M, Síma R, Tvrdík D, Povýsil C, Ryska A. KRAS mutation testing in therapeutic algorithm for treatment of metastatic colorectal carcinoma. Cas Lek Cesk. 2011;150(6):321-6.
• 52. Linos K, Sedivcová M, Cerna K, Sima R, Kazakov DV, Nazeer T, Glazyrin A, Valerian BT, Carlson JA. Extra nuchal-type fibroma associated with elastosis, traumatic neuroma, a rare APC gene missense mutation, and a very rare MUTYH gene polymorphism: a case report and review of the literature. J Cutan Pathol. 2011 Nov;38(11):911-8.
• 53. Kacerovská D, Michal M, Síma R, Grossmann P, Kazakov DV. Carney complex. Cesk Patol. 2011 Oct;47(4):192-7.

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