Job Position: Research Scientist
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Laboratory of Molecular Biology of Protists
Phone:
+420 387775481
E-mail:
horakova@paru.cas.cz
Room:
PARU II, 1. patro
EDUCATION
2000-2005
Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic
Ph.D. In Molecular Biology
Thesis :“Functional analysis of mitochondrial proteins in Trypanosoma brucei procyclics.“
supervisor: Prof. Julius Lukes, email: jula@paru.cas.cz
1995-2000
Faculty of Science, Masaryk University, Brno, Czech Republic
M.S. In Molecular Biology and Genetics
Thesis: „Typization of HLA antigens in CVID patients“
supervisor: MUDr. Tomas Freiberger Ph.D., email: tomf@vuzd.cz
WORK EXPERIENCE
2010-present
Postdoctoral Fellow, Laboratory of Molecular Parasitology, Institute of Parasitology, Biology Centre, ASCR, v.v.i.
„Posttranscriptional modifications of mitochondrial and cytosolic tRNAs in Trypanosoma brucei“
2009 – 2010
Postdoctoral Fellow, McMaster laboratory, Department of Medical Genetics, Vancouver Coastal Health Research Institute,
University of British Columbia, Jack Bell Research Centre
“Proteomic Profiling of Host-Pathogen interaction: The Macrophage response to Leishmania major” Prof. Robert W. McMaster (robm@interchange.ubc.ca)
2006-2008 Maternity leave
2000-2005
Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic.
Research Scientist in Laboratory of Molecular Parasitology
2003 6 month research stay in Prof. Kenneth Stuart laboratory, Seattle, Biomedical Research Institute, Seattle, Washington, USA
2002 6 month research stay in Dr. Rob Benne laboratory, Department of Biochemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, supported by EURESCO Exchange Grant given to E.V.
1999-2000
Department of Imunogenetics, Research Institute of Child Health, Brno, Czech Republic bachelor
Student of Molecular Biology and Genetics
Notable awards:
2007, Otto Wichterle Premium for young scientific team, AS CR
2005, honored PhD thesis “Functional analysis of mitochondrial proteins in Trypanosoma brucei procyclics”
2001, honored student grant “RNA interference in Trypanosoma brucei”
ACADEMICAL COURSES
“Grant Writing for Funding and Research Development” University of British Columbia
“Mass Spectrometry in Life Sciences,” University of British Columbia
“Proactive Communication & Effective Temwork Workshop” MITACS Skills
Training&Entrepreneurship Program (STEP)
SUPERVISION OF UNDERGRADUATE STUDENTS
2010 Melanie van Soeren: Characterization of the Leishmania protein GP63 using site direct mutagenesis
LABORATORY SKILLS
Wide range of general lab experience, focusing on molecular biology and proteomics:
Culture work (Leishmania ssp, Trypanosoma ssp.), Culture manipulation, Transfection studies, Macrophage infection studies, THP-1 cell cultivation, Peritoneal macrophage cell harvest.
Isolation of amastigotes from spleen/lesion, axenic cultivation of amastigotes
Leishmaniainfection rate. Limited Dilution assay. Spleen/Liver smears (H/E staining)
Viability assays (MTS assay, Alamar Blue assay)
Mass spectrometry sample preparation. Operating ABI 4800 mass spec, Protein Pilot, Statistical analysis of proteomics data.
Site-direct mutagenesis
Gelatin zymography
FACS analysis.
Isolation of DNA, RNA and mitochondrial vesicles
gel electrophoresis, SDS-PAGE,, 2D gel electrophoresis
Western and Northern blotting
Radiolabelling, Imunochemical staining
RNA interference of Trypanosoma brucei
immunofluorescent microscopy
PCR, RT-PCR, Quantitative RT-PCR, poisoned primer extension
in vitro editing
measurement of mitochondrial membrane potential
measurement of respiration rate
histochemical staining of protein complexes
NADH dehydrogenase, cytochrome creductase, and oxidase activity assays
Chmelová L., Kraeva N., Saura A., Krayzel A., Vieira C.S., Neves Ferreira T., Soares R.P., Bučková B., Galan A., Horáková E., Ganyukova A., Malysheva M.,
Butenko A.,
Prokopchuk G., Frolov A.,
Lukeš J., Horváth A.,
Škodová-Sveráková I., Feder D., Kostygov A., Yurchenko V. (2024) Intricate balance of dually-localized catalase in /Leptomonas seymouri/ (Kinetoplastea: Trypanosomatidae) modulates infectivity International Journal for Parasitology
54
: 391-400.
DOI: 10.1016/j.ijpara.2024.04.007
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Kachale A., Pavliková Z.,
Nenarokova A., Roithová A.,
Durante I., Miletínová P.,
Záhonová K.,
Nenarokov S.,
Votýpka J., Horáková E., Ross R.L., Yurchenko V., Beznosková P.,
Paris Z., Valášek L.S.,
Lukeš J. (2023) Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment Nature
613
: 751–758.
DOI: 10.1038/s41586-022-05584-2
Documents to download:
Kachale_2023_Nature (pdf)
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Horáková E., Lecordier L., Cunha P., Sobotka R., Changmai P., Langedijk C.J.M., Abbeele J.V.D., Vanhollebeke B.,
Lukeš J. (2022) Heme-deficient metabolism and impaired cellular differentiation as an evolutionary trade-off for human infectivity in Trypanosoma brucei gambiense Nature Communications
13
: 7075 .
DOI: 10.1038/s41467-022-34501-4
Documents to download:
Horakova_2022_NatComm (pdf)
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Kořený L.,
Oborník M., Horáková E., Waller R.,
Lukeš J. (2022) The convoluted history of haem biosynthesis Biological Reviews of the Cambridge Philosophical Society
97
: 141–162.
DOI: 10.1111/brv.1279
Documents to download:
Koreny_2022_Biol-Reviews (pdf)
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Paris Z.,
Svobodová M.,
Kachale A., Horáková E.,
Nenarokova A.,
Lukeš J. (2021) A mitochondrial cytidine deaminase is responsible for C to U editing of tRNATrp to decode the UGA codon in Trypanosoma brucei RNA Biology
14
: 278–286.
DOI: 10.1080/15476286.2021.1940445
Documents to download:
Paris_2021_RNA (pdf)
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Horáková E.,
Faktorová D., Kraeva N.,
Kaur B., Van Den Abbeele J., Yurchenko V.,
Lukeš J. (2020) Catalase compromises the development of the insect and mammalian stages of Trypanosoma brucei FEBS Journal
287
: 964–977.
DOI: 10.1111/febs.15083
Documents to download:
Horakova_2020_FEBSJournal (pdf)
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Podešvová L., Leštinová T., Horáková E.,
Lukeš J., Volf P.,
Yurchenko V. (2020) Suicidal Leishmania Pathogens
9
: 79.
DOI: 10.3390/pathogens9020079
Documents to download:
Podesvova_2020_Pathogens (pdf)
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Bianchi C., Kostygov A., Kraeva N., Záhonová K., Horáková E., Sobotka R.,
Lukeš J.,
Yurchenko V. (2019) An enigmatic catalase of Blastocrithidia. Molecular and Biochemical Parasitology
232
: 111199.
DOI: 10.1016/j.molbiopara.2019.111199
Documents to download:
Bianchi_Molecular & Biochemical Parasitology_2019 (pdf)
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Haindrich A., Boudová M.,
Vancová M.,
Pena Diaz C., Horáková E.,
Lukeš J. (2017) The intermembrane space protein Erv1 of Trypanosoma brucei is essential for mitochondrial Fe-S cluster assembly and operates alone Molecular and Biochemical Parasitology
214
: 47-51.
DOI: 10.1016/j.molbiopara.2017.03.009
Documents to download:
Haindrich et al. 2017 MBP (pdf)
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Horáková E.,
Changmai P.,
Vancová M., Sobotka R., Van Den Abbeele J., Vanhollebeke B.,
Lukeš J. (2017) The Trypanosoma brucei TbHrg protein is a heme transporter involved in regulation of stage-specific morphological transitions The Journal of Biological Chemistry
292
: 6998-7010.
DOI: 10.1074/jbc.M116.762997
Documents to download:
Horakova et al. 2017 (pdf)
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Kraeva N., Horáková E., Kostygov A.,
Kořený L.,
Butenko A.,
Yurchenko V.,
Lukeš J. (2017) Catalase in Leishmaniinae: With me or against me? Infection, Genetics and Evolution
50
: 121-127.
DOI: 10.1016/j.meegid.2016.06.054
Documents to download:
Kraeva et al. 2017 JBCh (pdf)
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Basu S., Horáková E.,
Lukeš J. (2016) Iron-associated biology of Trypanosoma brucei. Biochimica et Biophysica Acta
1860
: 368-370.
DOI: 10.1016/j.bbagen.2015.10.027
Documents to download:
Basu et al. 2016 B et B Acta (pdf)
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Horáková E.,
Changmai P.,
Paris Z., Salmon D.,
Lukeš J. (2015) Simultaneous depletion of Atm and Mdl rebalances cytosolic Fe-S cluster assembly but not heme import into the mitochondrion of Trypanosoma brucei FEBS Journal
282
: 4157-4175.
DOI: 10.1111/febs.13411
Documents to download:
Horkova et al. 2015 FEBS Journal (pdf)
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Sample P.,
Kořený L.,
Paris Z., Gaston K., Rubio M., Fleming I., Hinger S., Horáková E., Limbach P.,
Lukeš J., Alfonzo J. (2015) A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria Nucleic Acid Research
43
: 4262-4273.
DOI: 10.1093/nar/gkv286
Documents to download:
Sample_et_al_NAR_2015 (pdf)
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Verner Z.,
Basu S., Benz C.,
Dixit S.,
Dobáková E.,
Faktorová D.,
Hashimi H., Horáková E.,
Huang Z.,
Paris Z., Pena P., Ridlon L.,
Týč J.,
Wildridge D.,
Zíková A.,
Lukeš J. (2015) The malleable mitochondrion of Trypanosoma brucei Internation Review of Cell and Molecular Biology
315
: 73-151.
DOI: 10.1016/bs.ircmb.2014.11.001
Documents to download:
Verner-IRCMB-2015 (pdf)
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Kovářová J., Horáková E.,
Changmai P.,
Vancová M.,
Lukeš J. (2014) Mitochondrial and nucleolar localization of cysteine desulfurase Nfs and the scaffold protein Isu in Trypanosoma brucei Eukaryotic Cell
13
: 353-362.
DOI: 10.1128/EC.00235-13
Documents to download:
Kovarova-EC-2014 (pdf)
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Changmai P., Horáková E.,
Long S.,
Černotíková-Stříbrná E., Mcdonald L., Bontempi E.,
Lukeš J. (2013) Both human ferredoxins equally efficiently rescue ferredoxin deficiency in Trypanosoma brucei Molecular microbiology
89
: 135-151.
DOI: 10.1111/mmi.12264
Documents to download:
2013_Changmai_MM_135 (pdf)
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Paris Z., Horáková E., Rubio M., Sample P., Fleming I., Armocida S.,
Lukeš J., Alfonzo J. (2013) The T. brucei TRM5 methyltransferase plays an essential role in mitochondrial protein synthesis and function. RNA
19
: 649-658.
Documents to download:
2013_Paris_RNA_649 (pdf)
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Šmíd O., Horáková E., Vilímová V., Hrdý I., Cammack R., Horváth A.,
Lukeš J., Tachezy J. (2006) Knock-downs of mitochondrial iron-sulfur cluster assembly proteins IscS and IscU down-regulate the active mitochondrion of procyclic Trypanosoma brucei. Journal of Biological Chemistry
281
: 28679-28686.
DOI: 10.1074/jbc.M513781200
Documents to download:
Smid et al. 2006 JBCh (pdf)
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Zíková A., Horáková E.,
Jirků M., Dunajčíková P.,
Lukeš J. (2006) The effect of down-regulation of mitochondrial RNA-binding proteins MRP1 and MRP2 on respiratory complexes in procyclic Trypanosoma brucei. Molecular and Biochemical Parasitology
149
: 65-73.
DOI: 10.1016/j.molbiopara.2006.04.007
Documents to download:
Zikova et al. 2006 MBP (pdf)
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