RNDr. Jan Kamiš

Job Position: PhD student - Laboratory of Arbovirology

Contact details

Phone: +420 38 777 5463
E-mail: jan.kamis@paru.cas.cz
Web: https://klistatavemeste.vsb.cz/
Room: 324

Description

My research is focused on the field of eco-epidemiology, with a specific interest in zoonotic pathogens that are transmitted to humans via vertebrates and arthropods such as ticks and mosquitoes. The primary focus of my study is on viruses from the Flaviviridae family - Tick-borne encephalitis virus, Usutu virus, West Nile virus, Dengue virus, and Alongshan virus, the genus Orbivirus (Tribeč/Kemerovo virus), and the family Hantaviridae (DOBV, TULV, PUUV, SWSV). In addition, the study encompasses bacteria such as Borrelia, Anaplasma, Neoehrlichia, and Rickettsia genera. I am involved in several projects in the laboratory, with a particular focus on the project Ticks in the City.

In the laboratory, I specialise in sterile work with infectious agents in BSL2 and BSL3 conditions. I am experienced in the detection of viruses and bacteria - from the processing of the primal sample (dissection, homogenization, isolation of RNA/DNA) through molecular biology screening and virology methods (PCR, multiplex qPCR, RT-PCR, electrophoresis, plaque titration, virus neutralization tests, immunofluorescence, isolation of viruses in tissue cultures) to the pathogen identification (Sanger sequencing, WGS) and interpretation of phylogenetic realtionships. I am also skilled with tissue cultures, plasmids, and work in the BSL3 laboratory, where I perform experiments with viruses (SARS-CoV-2, West Nile virus, Dengue virus…) and mouse models. I also provide OSH, and BSL2/BSL3 biohazard training for our employees. 

Curriculum vitae

EDUCATION:

Bachelor's degree (2016 – 2020)

• Study program: Biology
• Faculty of Science, University of South Bohemia in České Budějovice
• Bachelor's thesis: Phylogenetic relationships of coccidia parasitizing striped field mice (Apodemus agrarius) based on sequences of the COX3 gene.

Master's degree (2020 – 2022)

• Study program: Parasitology
• Faculty of Science, University of South Bohemia in České Budějovice
• Master's thesis: Rodents as reservoirs of hantaviruses

Rigorous proceeding (10/2022)

• Field: Parasitology
• Faculty of Science, University of South Bohemia in České Budějovice
• Rigorous thesis: Orthohantaviruses in the reservoir and atypical hosts in the Czech Republic: spillover infection and indication of virus-specific tissue tropism

Doctoral study (2023 - present)

• Study program: Parasitology
• Faculty of Science, University of South Bohemia in České Budějovice
• Topic: Ecoepidemiology of recognized and potential zoonotic pathogens of human

 

CONFERENCES:
 

XXVII. Celostátní konference virologů, Hradecké virologické dny, 10. - 11. 9. 2025, Nové Adalbertinum, Hradec Králové, Czech Republic

• Talk - Flaviviry u klíšťat a pacientů České republiky

16th International Symposium on Ticks and Tick-borne Diseases (ISTTBD-XVI), 26. - 28. 3. 2025, Weimar, Germany

• Talk - Tick-borne viral pathogens in ticks and human patients from the Czech Republic

31. Pečenkovy epidemiologické dny, 11. - 13. 9. 2024, Plzeň, Czech Republic

• Talk - Klíšťata ve městě – kde číhá riziko bakteriální nákazy?

XXVI. Celostátní konference virologů, Hradecké virologické dny, 28. - 29. 5. 2024, Nové Adalbertinum, Hradec Králové, Czech Republic

• Talk - Klíšťaty přenášené virové patogeny u klíšťat a pacientů v Jihočeském kraji

CzechoSlovak Virology Conference, 15. - 16. 2. 2024, Česká zemědělská univerzita, Praha, Czech Republic

• Poster - Tick-borne encephalitis virus in patients and in ticks from natural endemic sites in South Bohemia

XXV. Celostátní konference virologů, Hradecké virologické dny, 17. - 18. 10. 2023, Nové Adalbertinum, Hradec Králové, Czech Republic

• Talk - Virus klíšťové encefalitidy u pacientů a klíšťat v Jihočeském kraji

National Institute of Virology and Bacteriology (NIVB) meeting, 2. - 5. 10. 2023, Kutná Hora, Czech Republic

• Poster - Tick-borne encephalitis virus in patients and in ticks from natural endemic sites in South Bohemia

The 2nd French – Czech Tick Meeting (FCTM2), 25. - 26. 9. 2023, Biologické centrum AV ČR, České Budějovice, Czech Republic

• Talk - Tick-borne bacterial diseases in urban environments - where does the real danger lurk?

XXIV. Celostátní konference virologů, Hradecké virologické dny, 4. - 5. 10. 2022, Nové Adalbertinum, Hradec Králové, Czech Republic

• Talk - Orthohantaviry v rezervoárových a atypických hostitelích v ČR

• Poster - Hantaviry v rezervoárových a atypických hostitelích na urbánních lokalitách České republiky - hostitelská specifita a tkáňový tropismus

Publications