RNDr. Jan Kamiš

Job Position: PhD student - Laboratory of Arbovirology

Contact details

Phone: +420 38 777 5463
E-mail: jan.kamis@paru.cas.cz
Web: https://klistatavemeste.vsb.cz/
Room: 324

Description

My journey in the Laboratory of Arbovirology started in 2019, as a master's degree student I studied orthohantaviruses in rodents. In 2021 I started to work as a member of the professional staff and since 2023 I have become a Ph.D. student.

I am interested in ecoepidemiology of recognised and potential zoonotic pathogens of human. I am closely involved in several ongoing projects in the laboratory, particularly in the project Tick-borne bacterial diseases in urban environments (also known as “Ticks in the City”) and the optimisation of TBEV detection in human patients. I focus on pathogenic viruses and bacteria in ticks and vertebrate hosts (rodents, insectivores, birds…), mainly on the viruses from the Flaviviradae family (TBEV, USUV), genus Orbivirus (Tribeč/Kemerovo virus), family Hantaviridae (DOBV, TULV, PUUV, SWSV) and others. From the bacterial world, I am interested in Borrelia, Anaplasma and Rickettsia genera.

In the laboratory, I specialise in sterile work with infectious agents in BSL2 and BSL3 conditions. I am experienced in the detection of viruses and bacteria - from the processing of the primal sample (dissection, homogenization, isolation of RNA/DNA) through molecular biolog screening methods (PCR, multiplex qPCR, RT-PCR, electrophoresis, plaque titration, isolation of viruses in tissue cultures) to the pathogen identification (Sanger sequencing, WGS) and interpretation of phylogenetic realtionships. I am also used to working with tissue cultures, plasmids, and in the BSL3 laboratory, I work with viruses (SARS-CoV-2, TBEV…) and mouse models. I also provide OSH, and BSL2/BSL3 biohazard training for our employees. 

Curriculum vitae

EDUCATION:

Bachelor's degree (2016 – 2020)

• Study program: Biology
• Faculty of Science, University of South Bohemia in České Budějovice
• Bachelor's thesis: Phylogenetic relationships of coccidia parasitizing striped field mice (Apodemus agrarius) based on sequences of the COX3 gene.

Master's degree (2020 – 2022)

• Study program: Parasitology
• Faculty of Science, University of South Bohemia in České Budějovice
• Master's thesis: Rodents as reservoirs of hantaviruses

Rigorous proceeding (10/2022)

• Field: Parasitology
• Faculty of Science, University of South Bohemia in České Budějovice
• Rigorous thesis: Orthohantaviruses in the reservoir and atypical hosts in the Czech Republic: spillover infection and indication of virus-specific tissue tropism

Doctoral study (2023 - present)

• Study program: Parasitology
• Faculty of Science, University of South Bohemia in České Budějovice
• Topic: Ecoepidemiology of recognized and potential zoonotic pathogens of human

 

CONFERENCES:

31. Pečenkovy epidemiologické dny, 11. - 13. 9. 2024, Plzeň

• Talk - Klíšťata ve městě – kde číhá riziko bakteriální nákazy?

XXVI. Celostátní konference virologů, Hradecké virologické dny, 28. - 29. 5. 2024, Nové Adalbertinum, Hradec Králové

• Talk - Klíšťaty přenášené virové patogeny u klíšťat a pacientů v Jihočeském kraji

CzechoSlovak Virology Conference, 15. - 16. 2. 2024, Česká zemědělská univerzita, Praha

• Poster - Tick-borne encephalitis virus in patients and in ticks from natural endemic sites in South Bohemia

XXV. Celostátní konference virologů, Hradecké virologické dny, 17. - 18. 10. 2023, Nové Adalbertinum, Hradec Králové

• Talk - Virus klíšťové encefalitidy u pacientů a klíšťat v Jihočeském kraji

National Institute of Virology and Bacteriology (NIVB) meeting, 2. - 5. 10. 2023, Kutná Hora

• Poster - Tick-borne encephalitis virus in patients and in ticks from natural endemic sites in South Bohemia

The 2nd French – Czech Tick Meeting (FCTM2), 25. - 26. 9. 2023, Biologické centrum AV ČR, České Budějovice

• Talk - Tick-borne bacterial diseases in urban environments - where does the real danger lurk?

XXIV. Celostátní konference virologů, Hradecké virologické dny, 4. - 5. 10. 2022, Nové Adalbertinum, Hradec Králové

• Talk - Orthohantaviry v rezervoárových a atypických hostitelích v ČR

• Poster - Hantaviry v rezervoárových a atypických hostitelích na urbánních lokalitách České republiky - hostitelská specifita a tkáňový tropismus

Publications