Tomáš Bílý, Ph.D.

Job Position: Postdoc - Laboratory of Electron Microscopy

Contact details

Phone: +420 387775444
E-mail: thomass@paru.cas.cz
Room: 405

Description

My main research interest is biological imaging by means of TEM.

Specifically, visualization of tissues, cells, purified organelles and viruses using techniques:
- TEM imaging and subsequent image processing and analyses.
- Electron tomography of room temperature samples and cryo-samples
- Sub Tomogram Averaging

Education:
-      PhD.       Biophysics
-      MSc.      Applied Measuring and Computer Engineering
-      Bc.         Measuring and Computer Engineering

Internships:
-      2014 Heidelberg – EMBL, Electron Microscopy Core Facility
-      2022 Heidelberg – Heidelberg University, Bioquant – Membrane biology of viral infection

I also do Light Microscopy, SEM, AFM and marginally Arduino-based tinkering that can be utilised in the LEM.

Publications
Total found: 23 records
Kitzberger F., Yang S., Týč J., Bílý T., Nebesářová J. (2024) An advanced fast method for the evaluation of multiple immunolabelling using gold nanoparticles based on low-energy STEM Scientific Reports 14 : 10150.
DOI: 10.1038/s41598-024-60314-0
Mashchenko O., Fessl T., Dyčka F., Bílý T., Vancová M., Kaftan D., Al-Muhkhrabi Y., Patlejchová T., Kratochvíl J. (2024) Laser metal printing from nanoparticles prepared by a gas aggregation source Nano Letters 44 : 14146–14152.
DOI: 10.1021/acs.nanolett.4c03131
Prančlová V., Nedvědová L., Kotounová E., Hönig V., Dvořáková M., Davídková M., Bílý T., Vancová M., Růžek D., Palus M. (2024) Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis Microbes and Infection 26 : 105383.
DOI: 10.1016/j.micinf.2024.105383
Tomasch J., Kopejtka K., Bílý T., Gardiner A.T., Gardian Z., Shivaramu S., Koblížek M., Kaftan D. (2024) A photoheterotrophic bacterium from Iceland has adapted its photosynthetic machinery to the long days of polar summer mSystems 9 : e01311-23.
DOI: 10.1128/msystems.01311-23
Vinopalová M., Arbonová L., Füssy Z., Dohnálek V., Samad A., Bílý T., Vancová M., Doležal P. (2024) Mlf mediates proteotoxic response via formation of cellular foci for protein folding and degradation in Giardia PLoS Pathogens 20 : e1012617 .
DOI: 10.1371/journal.ppat.1012617
Ďurinová E., Mojzeš P., Bílý T., Franta Z., Fessl T., Borodavka A., Tůma R. (2023) Chapter Five - Shedding light on reovirus assembly—Multimodal imaging of viral factories Advances in Virus Research 116 : 173–213.
DOI: 10.1016/bs.aivir.2023.06.002
Kafkova A., Tilokani L., Trčka F., Šrámková V., Vancová M., Bílý T., Nebesářová J., Prudent J., Trnka J. (2023) Selective and reversible disruption of mitochondrial inner membrane protein complexes by lipophilic cations Mitochondrion 68 : 60–71.
DOI: 10.1016/j.mito.2022.11.006
Muñoz-Gómez S.A., Cadena L., Gardiner A.T., Leger M.M., Sheikh S., Connell L.B., Bílý T., Kopejtka K., Beatty T., Koblížek M., Roger A., Slamovits C.H., Lukeš J., Hashimi H. (2023) Intracytoplasmic-membrane development in alphaproteobacteria involves the homolog of the mitochondrial crista-developing protein Mic60 Current Biology 33 : 1099 - 1111.
DOI: 10.1016/j.cub.2023.02.059

Documents to download:
Munoz-Gomez_2023_ICM (pdf)
Vancová M., Skoupý R., Ďurinová E., Bílý T., Nebesářová J., Krzyzanek V., Kolouch A., Horodyský P. (2022) Cathodoluminescence imaging of cellular structures labeled with luminescent iridium or rhenium complexes at cryogenic temperatures Scientific Reports 12 : 13432 .
DOI: 10.1038/s41598-022-17723-w
Bílý T., Sheikh S., Mallet A., Bastin P., Pérez-Morga D., Lukeš J., Hashimi H. (2021) Ultrastructural Changes of the Mitochondrion During the Life Cycle of Trypanosoma brucei. Journal of Eukaryotic Microbiology 68 : e12846.
DOI: 10.1111/jeu.12846

Documents to download:
Bily_2021_JEukMic (pdf)
Graña‑Miraglia L., Šikutová S., Vancová M., Bílý T., Fingerle V., Sing A., Castillo‑Ramírez S., Margos G., Rudolf I. (2020) Spirochetes isolated from arthropods constitute a novel genus Entomospira genus novum within the order Spirochaetales Scientific Reports 10 : 17053.
DOI: 10.1038/s41598-020-74033-9
Vancová M., Bílý T., Šimo L., Touš J., Horodyský P., Růžek D., Novobilský A., Salat J., Strnad M., Sonenshine D.E., Grubhoffer L., Nebesářová J. (2020) Three-dimensional reconstruction of the feeding apparatus of the tick Ixodes ricinus (Acari: Ixodidae): a new insight into the mechanism of blood-feeding Scientific Reports 10 : 165.
DOI: 10.1038/s41598-019-56811-2
Matoušková M., Bílý T., Bruňanská M., Oros M., Kostič B., Nebesářová J. (2019) New data on spermiogenesis and trepaxonematan axoneme in basal tapeworms (Cestoda, Caryophyllidea, Lytocestidae) parasiting cyprinid fishes Scientific Reports 9 : 12881.
DOI: 10.1038/s41598-019-49312-9
Vancová M., Bílý T., Nebesářová J., Grubhoffer L., Bonnet S., Šimo L. (2019) Ultrastructural mapping of salivary gland innervation in the tick Ixodes ricinus Scientific Reports 9 : 6860.
DOI: 10.1038/s41598-019-43284-6
Kaurov I., Vancová M., Schimanski B., Cadena L.R., Heller J., Bílý T., Potěšil D., Eichenberger C., Bruce H., Oeljeklaus S., Warscheid B., Zdráhal Z., Schneider A., Lukeš J., Hashimi H. (2018) The diverged trypanosome MICOS complex as a hub for mitochondrial cristae shaping and protein import Current Biology 28 : 3393–3407.
DOI: 10.1016/j.cub.2018.09.008

Documents to download:
Kaurov et al. 2018_Current Biology (pdf)
Matoušková M., Bílý T., Bruňanská M., Mackiewicz J., Nebesářová J. (2018) Ultrastructural, cytochemistry and electron tomography analysis of Caryophyllaeides fennica (Schneider, 1902) (Cestoda: Lytocestidae) reveals novel spermatology characteristics in the Eucestoda Parasitology Research 117 : 3091–3102.
DOI: 10.1007/s00436-018-6001-9
Štefánik M., Formanová P., Bílý T., Vancová M., Eyer L., Palus M., Salát J., Braconi C. T. , Zanotto De A. P., Gould E., Růžek D. (2018) Characterisation of Zika virus infection in primary human astrocytes. BMC Neuroscience BMC Neuroscience 18 : 1136.
DOI: 10.1186/s12868-018-0407-2
Hartigan A., Estensoro Atienza I., Vancová M., Bílý T., Patra S., Eszterbauer E., Holzer A. (2016) New cell motility model observed in parasitic cnidarian Sphaerospora molnari (Myxozoa:Myxosporea) blood stages in fish Scientific Reports 6 : 39093.
DOI: 10.1038/srep39093
Vávra J., Bílý T., Nebesářová J., Federici B. (2016) Occurrence, pathology, and ultrastructure of iridovirus and cytoplasmic polyhedrosis viruses in daphnids from the Czech Republic Journal of Invertebrate Pathology 140 : 35-38.
DOI: 10.1016/j.jip.2016.07.008
Bílý T., Palus M., Eyer L., Elsterová J., Vancová M., Růžek D. (2015) Electron tomography analysis of tick-borne encephalitis virus infection in human neurons Scientific Reports 5 : 10745.
DOI: 10.1038/srep10745

More publications

CONTACT

Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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